基于数据挖掘和网络针灸学探讨针灸治疗糖尿病前期核心腧穴的作用机制OA

Objective:To summarize and analyze the acupoint selection patterns of acupuncture and moxibustion for prediabetes using data mining and network acu-informatics,and to explore the action mechanisms of core acupoint combinations.Methods:Relevant studies indexed in CNKI,Wanfang,VIP,CBM,PubMed,Embase,Cochrane Library,and Web of Science were used as data sources.The acupoints involved in acupuncture and moxibustion prescriptions were analyzed in terms of usage frequency,relative frequency statistics,association analysis,and core acupoint combination extraction.Mechanistic exploration was performed based on the identified core acupoint combinations,including target prediction,construction of a'core acupoint-shared targets'network,protein-protein interaction(PPI)network analysis,and KEGG and GO enrichment analyses,to investigate the potential mechanisms of acupuncture and moxibustion in treating prediabetes.Results:A total of 100 acupuncture prescriptions were included.The core acupoint combination consisted of Sanyinjiao(SP6),Zusanli(ST36),Pishu(BL20),Fenglong(ST40),and Weiwanxiashu(EX-B3).A total of 96 potential therapeutic targets were identified,of which 50 overlapped with targets associated with prediabetes.PPI network analysis revealed key targets,including interleukin-6(IL-6),signal transducer and activator of transcription 3(STAT3),and interleukin-1β(IL-1β).KEGG and GO enrichment analyses indicated that the core acupoint combination modulates signaling pathways related to adipocytokines,non-alcoholic fatty liver disease,lipid metabolism and atherosclerosis,alcoholic liver disease,and insulin resistance.It is involved in regulating biological processes such as carbohydrate metabolism,insulin response,peptide hormone response,oxygen level response,small-molecule metabolic processes,and cellular response to lipids.Conclusion:The core acupoint combination of acupuncture and moxibustion treatment for prediabetes includes SP6,ST36,BL20,ST40,and EX-B3.This combination exerts therapeutic effects on prediabetes through multi-target and multi-pathway synergistic mechanisms.Additionally,it may offer advantages in the comprehensive management of metabolic syndrome manifestations such as obesity,fatty liver,and dyslipidemia.