大花红景天盐胁迫调控基因RcNAC22克隆及功能分析OA

This study cloned the RcNAC22 gene of R.crenulata and conducted bioinformatics analysis and functional verification.The results showed that the full length of RcNAC22 gene was 1 049 bp,containing two introns and three exons,and encoding 293 amino acids.Its protein relative molecular weight was 33 625.02,belonging to a hydrophilic protein,with relatively stable properties,no transmembrane domain or signal peptide,and subcellular localization in the nucleus,containing 32 phosphorylation sites.RcNAC22 contains one NAM domain(no apical meristem),and the fourth domain is the transcriptional repression domain NARD region,and it was most closely related to that of R.kirilowii.RcNAC22 gene was expressed in all six tested organs(root,mature stem,young stem,leaf,apical bud,flower)but mainly in green organs(stem,leaf,apical bud),and its expression could be induced by various abiotic stresses and plant hormones ABA and SA.RcNAC22 gene had no cytotoxicity to yeast cells,and both the full-length gene and truncated C-terminal had self-activation activity.In addition,using Salvia miltiorrhiza as the transgenic receptor to study the RcNAC22 gene effect on plant growth,the results showed that overexpression of RcNAC22 gene did not affect the growth and development of Salvia miltiorrhiza,but reduced the tolerance to salt stress.This study revealed the biological function of the RcNAC gene,providing a theoretical basis for further molecular biological research on R.crenulata.