黑果枸杞LrMYB64基因克隆及功能研究OA

Lycium ruthenicum,a characteristic food-medicine plant in northwestern China,accumulates abundant anthocyanins in its fruits and possesses high pharmaceutical value.In order to identify the key regulators of anthocyanin biosynthesis and to clarify their coupled effects on lateral-bud development,the candidate gene LrMYB64 was cloned,an overexpression vector was constructed and transformed into L.ruthenicum,and the underlying molecular network was explored using transcriptome sequencing.The results showed that overexpression of LrMYB64 gene in L.ruthenicum significantly suppressed the expression of key structural genes in the anthocyanin biosynthetic pathway-including LrCHS1B,LrCHS2,LrF3H,LrDFR,LrANS,and LrA3GT-leading to the reduction by 91.75％ in anthocyanin level.Meanwhile,it markedly upregulated multiple hormone-signaling genes such as LrPR4,LrPP2C51,LrSAUR36,LrGH3.6,LrARF5,and LrABF4,increasing lateral bud outgrowth to approximately three times that of the wild type.Together,these findings indicated that LrMYB64 gene orchestrated a gene regulatory network through which it repressed anthocyanin biosynthesis while promoting hormone signal transduction,thereby coordinating plant secondary metabolism and hormonal responses.