原花青素B2对地塞米松诱导骨髓间充质干细胞成骨分化的影响OA

Objective:To investigate the effect of procyanidin B2 on dexamethasone-induced osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)via adjusting the Hippo/Yes-associated protein(YAP)pathway.Meth-ods:BMSCs cells were isolated from rats,identified and cultured in vitro.The cells were then divided into control group,dexam-ethasone group,dexamethasone+low,medium,and high dose procyanidin B2 groups,and dexamethasone+high dose procyanidin B2+YAP activator PY-60(dexamethasone+high dose procyanidin B2+PY-60)group.MTT assay was performed to detect cell viability.The ALP detection kit was used to measure ALP activity.Alizarin Red S staining was performed to visualize and quantify mineralized nodules in cells.Moreover,Western Blot was used to measure the expression levels of Runx2,OCN,Collagen Ⅰ,and Hippo/YAP pathway-related proteins in cells.Results:BMSCs were successfully isolated and cultured.Compared with the control group,the dexamethasone group showed a decreased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while increased p-YAP/YAP ratio(P<0.05).Compared with the dexamethasone group,the dexamethasone+low,medium,and high dose procyanidin B2 groups showed increased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while decreased p-YAP/YAP ratio(P<0.05).Compared with the dexamethasone+high dose procyanidin B2 group,the dexamethasone+high dose procyanidin B2+PY-60 group showed decreased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while increased p-YAP/YAP ratio(P<0.05).Conclusion:Pro-cyanidin B2 alleviates dexamethasone-induced inhibition of osteo-genic differentiation in BMSCs by inhibiting the Hippo/YAP pathway.