茯苓拮抗咖啡中枢兴奋作用的研究OA

Objective To explore the excitatory effects of different concentrations of coffee on the central nervous system and the antagonistic effects of the Poria cocos on the excitatory effects of coffee.Methods Wild-type Canton S female Drosophila melanogaster were divided into a blank group and 5 coffee groups(the concentrations of coffee were 0.125%,0.25%,0.25%,1%,and 2%respectively).Additionally,another blank group,12,24 and 48 h groups were established separately.Taking total daily autonomous activity and total daily sleep time as metrics to reveal the dose-effect and time-effect relationships of coffee on Drosophila excitation.Thereby the best concentrations of coffee and optimal administration time were selected.New cohorts of Drosophila were chosen and randomly divided into the four groups:blank group,model group,Poria cocos group and diazepam group,to further investigate the intervention of Poria cocos in coffee-induced alterations of Drosophila autonomous activity,sleep time,and sleep rhythm.Results 1%coffee administration for 12 h could significantly reduce the sleep time and increase the number of autonomous activities of Drosophila(P<0.01,P<0.05).Compared with the blank group,the model grow group demonstrated a significant increase in total daily,daytime,and nighttime autonomous activity(P<0.01),a significant decrease in sleep time(P<0.01,P<0.05).Compared with the model group,Poria cocos group and diazepam group significantly reduced coffee-induced autonomous activity and increased total sleep time in Drosophila(P<0.01,P<0.05).Conclusion Coffee has strong central nervous system excitatory effects at relatively lower concentrations,while Poria cocos can antagonize the excitatory effects of coffee.