ADIPOQ基因多态性与策勒黑羊、哈萨克羊和多浪羊生长性状的关联分析OA

This study was conducted to investigate the effects of ADIPOQ gene polymorphism on the growth traits of three local sheep breeds in Xinjiang namely Qira Black sheep,Kazakh sheep and Duolang sheep,so as to provide novel genetic marker resources for the molecular breeding of growth traits in Xinjiang sheep.A total of 560 sheep individuals,including 173 Qira Black sheep,186 Kazakh sheep and 201 Duolang sheep,were selected as the research objects.Phenotypic data including body height,body weight,body length and chest circumference of the three sheep breeds at one year of age were collected.Sanger sequencing combined with PCR-SSCP technology was used to detect the single nucleotide polymorphisms(SNPs)of ADIPOQ gene,and the association analysis between these SNPs and growth traits was carried out.The results showed that a missense mutation of g.201202805G＞T was detected in the exon 1 of ADIPOQ gene,and this mutation site had three genotypes named GG,GT and TT.The frequence of the three genotypes were 0.34,0.55 and 0.11 in Kazakh sheep,0.36,0.54 and 0.10 in Duolang sheep,and 0.34,0.57 and 0.09 in Qira Black sheep.GT was the dominant genotype and G was the dominant allele in all the three breeds.Association analysis results indicated that in Kazakh sheep and Duolang sheep populations,the body weight,body length,body height and chest circumference of individuals with GT genotype were significantly higher than those with GG and TT genotypes(P＜0.05).In Qira Black sheep population,the body weight,body length and chest circumference of individuals with GT genotype were significantly higher than those with GG and TT genotypes(P＜0.05),while there was no significant difference in body height among individuals with different genotypes(P＞0.05).In conclusion,the ADIPOQ gene plays an important role in regulating the growth traits of Qira Black sheep,Kazakh sheep and Duolang sheep,and its g.201202805G＞T mutation site can serve as a potential molecular genetic marker for evaluating the growth traits of these sheep breeds.