基于生物信息学筛选丹参酮ⅡA作用于心肌梗死的关键靶点OA

Objective Based on bioinformatics methods,the key targets of tanshinone ⅡA in treating myocardial infarction(MI)were systematically screened,and the molecular mechanism was explored.Methods The MI-related transcriptome data sets(GSE62646,GSE83500)were obtained from the Gene Expression Omnibus database,and integrate the TCMSP,CTD,and SwissTargetPrediction databases to obtain the potential targets of tanshinone ⅡA.The intersecting genes were extracted through Venn diagrams and a protein-protein interaction(PPI)network was constructed.Functional enrichment analysis,including Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses,was performed using DAVID.Molecular docking was conducted using CB-Dock2 to validate binding affinity.Results A total of 8 core candidate gene targets were identified,including SCN9A,PIM1,GSK3A,RELA,HMGB1,FASN,ACAT1 and MPI.Molecular docking showed that the binding energies of tanshinone ⅡA with all targets were lower than ˗6.8kcal/mol,with the strongest binding observed for SCN9A(˗10.6kcal/mol)and PIM1(˗10.2kcal/mol).GO analysis revealed that the targets were significantly enriched in biological processes such as inflammatory response and interleukin regulation.KEGG analysis indicated that the main pathways were fatty acid metabolism,the advanced glycation end product/advanced glycation end product receptor signaling pathway,and the acute myeloid leukemia pathway.Conclusion Tanshinone ⅡA may exert multi-dimensional protective effects in the treatment of MI by acting on multiple targets such as SCN9A and PIM1,and by synergistically regulating key pathways including inflammatory response and fatty acid metabolism.