Gata1/FLI1重编程B淋巴细胞生成巨核细胞样细胞OA

Platelet shortages are a common issue in clinical practice,and the growing demand for platelet transfusions has made this problem even more prominent.Platelets generated through cell reprogramming may provide a promising solution to address this critical challenge.Here,we report that enforced expression of transcription factors Gata1 and FLI1 reprograms murine bone marrow B lymphocytes into megakaryocytes.Notably,the expression of Gata1 alone in B lymphocytes was sufficient to induce the activation of megakaryocyte-specific markers,such as Cd41 and Cd42.Co-expression of FLI1 or treatment with the Ezh2/Ezh1 inhibitor UNC1999 significantly enhanced reprogramming efficiency.After 10-14 days,the reprogrammed cells acquired megakaryocyte morphology,and some cells proceeded to form pro-platelets.Importantly,we found that B lymphocytes retain their plasticity even after immortalization,this maybe the reason that Gata1/FLI1 could reprogram both primary and BCR-ABL-immortalized B lymphocytes.These findings suggest that B lymphocytes are a promising starting cell type for generating platelets through cell reprogramming and may be more suitable for large-scale production compared to adherent cells due to their suspension growth characteristics.