黄芪肉桂饮中化学成分鉴定及糖化酶活力测定OA
Identification of Chemical Components and Determination of Glucosidase Activity of Huangqi Rougui Decoction
目的:基于超高效液相色谱-四极杆-飞行时间串联质谱法(UPLC-Q/TOF-MS)对黄芪肉桂饮中化学成分进行快速鉴定,基于高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)对黄芪肉桂饮中糖化酶活力进行测定.方法:采用Waters Acquity UPLC BEH C18 色谱柱(100 mm×2.1 mm,1.7 μm),以乙腈-0.1%甲酸水溶液为流动相梯度洗脱,采用电喷雾离子源,正、负离子模式扫描,采集黄芪肉桂饮中化学成分的质谱信息.通过文献检索结果建立黄芪、肉桂中化学成分的数据库.基于UNIFI软件对采集的黄芪肉桂饮中化学成分的相对分子质量、二级碎片离子信息与文献检索数据进行比对,对黄芪肉桂饮中化学成分进行鉴定,分析其发酵后可能产生的单糖.采用Prevail Carbohyrate ES色谱柱(250 mm×4.6 mm,5 μm),以乙腈-水(75∶25)为流动相,柱温为 30℃,流速为1.0 mL·min-1,ELSD漂移管温度为 115℃,气体流速为 3 L·min-1,测定黄芪肉桂饮中糖化酶活力.结果:共鉴定了黄芪肉桂饮中 17 个化学成分.当黄芪肉桂饮中D-无水葡萄糖质量浓度为 114.9～1 148.9 μg·mL-1 时线性关系良好(r=0.999 1),其检测限为 30 μg·mL-1、定量限为 50 μg·mL-1,5 批黄芪肉桂饮中糖化酶活力分别为 27.97、29.53、29.20、31.32、29.05 U·μmol-1.结论:采用UPLC-Q/TOF-MS结合UNIFI软件可快速鉴定黄芪肉桂饮中化学成分,通过HPLC-ELSD可有效测定黄芪肉桂饮中糖化酶活力.
Objective:To establish an ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q/TOF-/MS)method for the rapid characterization of chemical components in Huangqi Rougui Decoction and measure its glucosidase activity using the high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method.Methods:A Waters Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm)was used for gradient elution with acetonitrile and 0.1%formic acid aqueous solution.MS data were collected through the utilization of electrospray ionization(ESI)in both positive and negative ion modes.The chemical component databases of Astragali Radix and Cinnamomi Cortex were established based on literature review.The UNIFI platform was used to compare the molecular weights and MS² fragment ion information of the chemical components in Huangqi Rougui Decoction with the established database for component identification,and the possible monosaccharides produced after fermentation were analyzed.The glucosidase activity in Huangqi Rougui Decoction was determined via a Prevail Carbohydrate ES column(250 mm×4.6 mm,5 μm)with the mobile phase consisting of acetonitrile and water at a ratio of 75:25,the column temperature of 30℃,and the flow rate of 1.0 mL·min-1.For the ELSD detector,the drift tube temperature was 115℃and the gas flow rate was 3 L·min-1.Results:A total of 17 compounds were identified in Huangqi Rougui Decoction.The HPLC-ELSD method demonstrated good linearity for D-glucose within the concentration range of 114.9-1 148.9 μg·mL-1(r=0.999 1).The limit of detection and limit of quantification were 30 μg·mL-1 and 50 μg·mL-1,respectively.The glucosidase activities of the five batches of Huangqi Rougui Decoction samples were 27.97,29.53,29.20,31.32,and 29.05 U·μmol-1,respectively.Conclusion:UPLC-Q/TOF-MS combined with the UNIFI platform allowed the rapid identification of the chemical components in Huangqi Rougui Decoction and the HPLC-ELSD method was proved effective for the measurement of glucosidase activity in Huangqi Rougui Decoction.
武哲;武浩楠;李明华;邱淦清;程显隆;魏锋
中国食品药品检定研究院 药品监管科学全国重点实验室,北京 102629||中国食品药品检定研究院 中药民族药检定研究所,北京 102629中国食品药品检定研究院 中药民族药检定研究所,北京 102629||沈阳药科大学 功能食品与葡萄酒学院,辽宁 沈阳 110016中国食品药品检定研究院 药品监管科学全国重点实验室,北京 102629||中国食品药品检定研究院 中药民族药检定研究所,北京 102629北京市东城区中贝中医药研究所,北京 100010中国食品药品检定研究院 药品监管科学全国重点实验室,北京 102629||中国食品药品检定研究院 中药民族药检定研究所,北京 102629中国食品药品检定研究院 药品监管科学全国重点实验室,北京 102629||中国食品药品检定研究院 中药民族药检定研究所,北京 102629
医药卫生
糖化酶活力黄芪肉桂饮蒸发光散射检测器法
glucosidase activityHuangqi Rougui Decoctionevaporative light scattering detector
《中国现代中药》 2026 (3)
605-613,9
国家重点研发计划项目(2023YFC3504105)
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