黑木耳多糖对蛋鸡饲喂效果的研究OA

In order to explore the dose-effect relationship and mechanism of Auricularia auricula polysaccharide(AAP)on laying hens in late laying period,400 56-week-old Hy-Line brown laying hens were randomly divided into basic diet group(C0 group)and 100,200,300 mg/kg AAP addition groups(group T1,group T2,group T3).The results showed that the laying rate,eggshell strength and eggshell color score in group T3 were increased by 8.9％,20.9％and 20.6％,respectively compared with group CO,and the feed-egg ratio were decreased by 7.4％(P＜0.05).AAP enhanced the antioxidant function by activating the Nrf2/ARE pathway.The activities of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)in group T3 were increased by 32.0％and 32.7％,respectively comprred with group CO,and the content of malondialdehyde(MDA)was decreased by 34.1％(P＜0.05).AAP was selective in immune regulation,and the levels of IL-2,IFN-γ and IL-10 in group T3 were significantly increased by 55.8％,81.9％and 123.3％,respectively(P＜0.05),but had no significant effect on IL-6.In addition,AAP improved egg quality by improving the eggshell matrix mineralization process.This study demonstrated the triple synergistic mechanism of AAP in laying hens production,and determined 300 mg/kg as the optimal addition amount,which provided important technical support for the development of new functional feed additives and antibiotic-free breeding practice.