辣椒果皮中细胞壁成分与XTH活性的差异分析OA

Fruit pericarp thickness in pepper is closely associated with cell wall components and xyloglucan endotransglucosylase/hydrolase(XTH)enzyme activity,yet relevant research remains insufficient.This study measured the contents of cellulose,hemicellulose,and pectin,as well as XTH activity,in pericarp tissues of five pepper inbred lines(three thick-pericarp pepper and two thin-pericarp pepper)at 10 and 35 days after pollination(DAP),and performed comparative and correlation analyses.The results showed that principal component analysis(PCA)indicated the first three principal components cumulatively explained 95.39％of the variance,revealing significant differences in metabolite accumulation patterns between the two pepper types.Quantitative analysis demonstrated that total pectin,cellulose,and hemicellulose contents were all higher in thin-pericarp peppers than in thick-pericarp peppers,and the contents of these three components were generally lower at 35 DAP compared to 10 DAP across the five pepper lines.Conversely,XTH activity was relatively higher in thick-pericarp peppers and was generally elevated at 35 DAP.Further correlation analysis revealed significant positive correlations among pectin,cellulose,and hemicellulose,while showing negative correlations with XTH activity.These findings indicate that cultivar type differences are important factors causing variations in cell wall components and XTH activity,providing a theoretical basis for improving pepper fruit quality through the regulation of cell wall homeostasis.