兰州百合远缘杂交亲和性及后代真实性检验OA

[Objective]This study aims to investigate hybrid affinity between Lilium davidii var.unicolor and L ilium ornamental cultivars,and to provide germplasm resources and technical support for breeding multi-purpose lilies.[Method]Pollen viability of all tested lilies was examined.Based on the results,10 cross combinations were established:using L.davidii var.unicolor as the male parent with'Tiger Babies''Purple Dream''Black Charm''Sweet Sugar''Eye Liner''Gizmo'and'Watch Up'as female parents,and using L.davidii var.unicolor as the female parent with'Purple Dream''Black Charm'and'Watch Up'as male parents.3 pollination methods were employed,including direct pollination,cut-style pollination,and stigma application with 1 g·L-1 NAA solution.Cross-compatibility was evaluated based on pollen tube growth and fruit set.The authenticity of hybrid seedlings obtained through embryo culture was assessed using SSR markers.[Result](1)Cross-compatibility analysis revealed that'Sweet Sugar'and'Black Charm'exhibited the highest compatibility with L.davidii var.unicolor,followed by'Eye Liner''Tiger Babies'and'Purple Dream'.In contrast,'Watch Up'and'Gizmo'showed low compatibility.(2)Direct pollination was the simplest and most universally applicable method.(3)SSR analysis revealed that all 145 seedlings from the 6 hybrid combinations('Sweet Sugar'×L.davidii var.unicolor,'Black Charm'×L.davidii var.unicolor,'Tiger Babies'×L.davidii var.unicolor,'Eye Liner'×L.davidii var.unicolor,L.davidii var.unicolor×'Black Charm',and L.davidii var.unicolor×'Purple Dream')were authentic hybrids.[Conclusion]The study revealed distinct cross-compatibility between L.davidii var.unicolor and different lily cultivars,identifying'Sweet Sugar'and'Black Charm'as highly compatible parents.Cut-style pollination is an effective technique for improving fruit set in the cross of'Tiger Baby'×L.davidii var.unicolor.SSR marker technology enables early and accurate identification of hybrid seedlings.[Ch,2 fig.6 tab.34 ref.]