3种黄连木属植物叶绿体基因组结构特征及遗传关系OA

[Objective]In this study,the chloroplast genomes of 3 Pistacia species were compared to analyze their structural characteristics and genetic evolution.[Method]3 publicly available chloroplast genomes of Pistacia species from National Center for Biotechnology Information(NCBI)were selected as research materials,and their chloroplast genome structure,repeat sequence,nucleotide polymorphism and genetic relationship were analyzed by relevant bioinformatics methods.[Result]The chloroplast genomes of the 3 species were tetrad ring structure,and the numbers of protein-coding sequence(CDS),ribosomal RNA(rRNA),and transfer RNA(tRNA)were exactly the same among them;The simple repeat sequences were mainly A(34.35%)or T(39.69%)repeat units in mono-nucleotide,but no hexa-nucleotide.And the number of scattered r epeat sequences were mainly palindromic repeats(105)and forward repeats(76),but no reverse repeats;There were different degrees of variation in the conserved non-coding sequences(CNS)of the large single copy(LSC)and small single copy(SSC)of the chloroplast genomes of three species,but there was no significant difference in the contraction or expansion of genes near the tetrad boundary;5 highly variable sequences(matK,trnG-UCC～trnR-UCU,trnT-UGU～trnL-UAA,petD～rpoA,rpl22～rpl2)were identified in the LSC region and the boundary between LSC and inverted repeat b(IRb);Phylogenetic reconstruction based on complete chloroplast genomes demonstrated clear segregation between Pistacia and Rhus,and P.chinensis was closely related to P.weinmaniifolia,while P.vera and P.atlantica could be formed a distinct clade.[Conclusion]The chloroplast genome structure of the 3 Pistacia species was similar and relatively conservative,and the number of various genes was consistent;The 5 mutant sequences detected could be used as candidate molecular markers for Pistacia species;The genetic relationship between P.chinensis and P.weinmannifolia was similar,while P.vera was relatively distant from former 2 species.[Ch,6 fig.1 tab.46 ref.]