一株凝结芽孢杆菌的益生菌性能评价及产乳酸条件优化OA
Evaluation of probiotic performance and optimization of lactic acid production conditions of Bacillus coagulans
本研究对凝结芽孢杆菌的益生菌性能进行评价,同时对其产酸条件进行优化.结果表明:该菌株在温度为80 ℃,pH为2,牛胆盐浓度为0.3％的条件下存活率能够达到40％,而且对人工胃液和肠液表现良好的耐受性.在产酸条件的优化中发现,在培养基成分为葡萄糖15 g/L、牛肉膏15 g/L、蛋白胨15 g/L、磷酸氢二钾6 g/L,培养温度40 ℃、初始pH 7.7、接种量0.5％、摇床转速180 r/min条件下发酵48 h时,其乳酸产量可以达到16.36 g/L.综上,该菌株具有良好的益生菌性能和产乳酸能力,在微生态制剂的制备与应用中有广泛应用前景.
In this study,the probiotic performance of Bacillus coagulans was evaluated,and the acid production conditions were optimized.The results showed that the survival rate of the strain could reach 40％at a temperature of 80 ℃,pH 2,and a bovine bile salt concentration of 0.3％,and it showed good tolerance to artificial gastric and intestinal juices.In the optimization of acid production conditions,it was found that the lactic acid yield could reach 16.36 g/L when fermented for 48 hours under the conditions of medium composition glucose 15 g/L,beef paste 15 g/L,peptone 15 g/L,dipotassium phosphate 6 g/L,culture temperature 40 ℃,initial pH 7.7,inoculum amount 0.5％,and shaker speed of 180 r/min.Therefore,this strain had good probiotic properties and lactic acid production ability and had a wide application prospect in the preparation and application of microecological preparations.
许颖;王红英;钱斯日古楞;王宝;赵治伟
大连工业大学生物工程学院,辽宁大连 116034大连工业大学生物工程学院,辽宁大连 116034大连工业大学生物工程学院,辽宁大连 116034乌兰煤炭(集团)有限责任公司,内蒙古鄂尔多斯 017200乌兰煤炭(集团)有限责任公司,内蒙古鄂尔多斯 017200
农业科技
微生态制剂益生菌性能凝结芽孢杆菌产酸条件优化人工胃液
microecological agentsprobiotic propertiesBacillus coagulansoptimization of acid production conditionsartificial gastric juice
《中国饲料》 2026 (7)
72-79,8
内蒙古自治区科技重大专项项目(222002023059)
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