SlProT1基因克隆、生物信息学分析及亚细胞定位OA

ProT is a type of amino acid transporter located on the plasma membrane,belonging to the AAAP family,responsible for the transport of amino acids from the apoplast to the cytosol.This experiment cloned and performed bioinformatics analysis and localization analysis on the protein encoded by SlProT1.The results showed that the SlProT1 encodes 441 amino acids,with a molecular weight of 48.54 Kda and a theoretical isoelectric point of 9.34.It is a stable hydrophobic protein with a protein structure including alpha helices,random coils,and extended strands.Real time fluorescence quantitative analysis showed that the SlProT1 is expressed in the root,stem,leaf,and flower tissues of tomatoes,with the highest expression level in the leaves,significantly higher than in the roots,stems,and flowers.Bioinformatics prediction shows that SlProT1 may be located on the plasma membrane and vacuolar membrane,while subcellular localization of SlProT1-mCherry comfirmed that SlProT1 is located on the plasma membrane.