染色体臂置换系CASL3AL早抽穗候选基因分析OA

[Objective]Heading date of Triticum aestivum is a key agronomic trait determining yield and quality.T.aestivum'Chinese Spring'(CS)-T.turgidum var.dicoccoides chromosome arm substitution line CASL3AL exhibits early heading under multi-environment cultivation.This study investigates the regulatory genes during critical stages of young spike development through transcriptome analysis.[Method]This study utilized recombinant inbred lines(RILs)derived from the cross between the wild emmer wheat chromosome arm substitution line CASL3AL and CS as materials,screening stable early-maturing lines(1017-7-167-1 and 1017-10-311-15-5)and the late-maturing parent CS.Main stem leaves at the single ridge stage(W1.5)and double ridge stage(W2.5)were collected for transcriptome sequencing(RNA-Seq).[Result]The homozygous s ingle nucleotide polymorphism(SNP)loci of 2 early-maturing lines were predominantly enriched in chromosome 3A.Differential expression analysis revealed:4 172 differentially expressed genes(DEGs)were identified at single ridge stage and 169 at double ridge stage in line 1017-7-167-1,while 1 065 and 172 DEGs were detected in line 1017-10-311-15-5 at corresponding stages.Gene ontology(GO)enrichment analysis indicated these DEGs were primarily involved in biological processes such as circadian rhythm and floral organ development.Based on SNP enrichment results and expression differences,2 candidate genes(TraesCS3A02G432900 and TraesCS3A02G139300)were identified within the SNP-enriched region of chromosome 3A.[Conclusion]This study identified candidate genes from wild emmer wheat that promote early heading in common wheat,providing a theoretical foundation for wheat growth period improvement.[Ch,9 fig.1 tab.32 ref.]