过表达紫花苜蓿ChOMT基因创制高异黄酮营养强化番茄OA

[Objective]This study aims to construct transgenic homoisoflavones tomato(Solanum lycopersicum)plants based on the homoisoflavones synthase gene ChOMT from alfalfa(Medicago sativa),thereby achieving nutritional fortification of tomatoes.[Method]ChOMT gene was introduced into tomatoes using agrobacterium-mediated transformation.Transgenic identification was carried out through PCR and fluorescence detection.Furthermore,plant-wide targeted metabolomics technology and multivariate statistical analysis methods were employed for precise quantitative analysis of homoisoflavones content.[Result]Through PCR and fluorescence detection,positive transgenic plants of ChOMT were obtained.Compared with the wild type,the content of homoisoflavonoids significantly increased.Among them,the relative content of(3RS)-5,7-dihydroxy-3-(2-hydroxy-4-methoxybenzyl)-chroman-4-one(HIF1)in the leaves of transgenic tomatoes was 57 019,which was 260 times higher than the relative content of 220 in the leaves of the wild type.T he relative content of 5,7-dihydroxy-3-(4-hydroxybenzyl)-chroman-4-one(HIF2)in the fruits of transgenic tomatoes was 15 849 985,which was over 4 000 times higher than the relative content of 3 849 in the wild type.[Conclusion]ChOMT transgenic tomatoes are obtained,which not only provides a theoretical basis for analyzing the catalytic function of ChOMT in plants,but also lays an important foundation of germplasm resources for breeding genetically improved tomato varieties with high homoisoflavonoid content.[Ch,6 fig.28 ref.]