超声微泡介导miR-545-3p调节E2F3信号通路对甲状腺癌细胞增殖、迁移、侵袭的影响OA

Objective To investigate the effects of ultrasound microbubble(UM)-mediated microRNA-545-3p(miR-545-3p)on the proliferation,migration,and invasion of thyroid cancer(TC)cells by regulating the E2F transcription factor 3(E2F3)signaling pathway.Methods mRNA levels of miR-545-3p and E2F3 were detected in PTC cell lines(TPC-1,K1,TTA1)and TC tissues by quantitative reverse transcription-polymerase chain reaction.The relationship between miR-545-3p and E2F3 was detected by dual luciferase assay.Microbubbles were prepared using ultrasound technology,and TPC-1 cells were assigned to control,miR-negative control(NC),miR-545-3p-mimics,UM+miR-545-3p-mimics,UM+miR-545-3p-mimics+pcDNA,and UM+miR-545-3p-mimics+E2F3 groups.The effects of ultrasound microbubble-mediated miR-545-3p on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of PTC cells by targeting E2F3 were detected.Expression levels of relevant proteins were detected by immunoblotting.Tumor transplant experiments were used to detect the effects of miR-545-3p on tumor growth and E2F3 expression.Results Expression levels of E2F3 mRNA were increased in TC tissues and TPC-1 cells,while expression levels of miR-545-3p decreased(P<0.05).Online prediction of the Starbase database found that there was a targeted binding site between miR-545-3p and E2F3.The number of cell clones,scratch-healing rate,and number of invasive cells were lower in the miR-545-3p-mimics group compared with the miR-NC group,expression levels of E2F3,cyclin-dependent kinase(CDK)1,matrix metalloproteinase(MMP)-2,MMP-9,N-Cadherin,and Vimentin were lower,while expression levels of miR-545-3p and E-Cadherin were higher(P<0.05).The number of cell clones,scratch-healing rate,and number of invasive cells were lower in the UM+miR-545-3p-mimics group compared with the miR-545-3p-mimics group,expression levels of E2F3,CDK1,MMP-2,MMP-9,N-Cadherin,and Vimentin were lower,while expression levels of miR-545-3p and E-Cadherin were higher(P<0.05).Compared with the UM+miR-545-3p-mimics+pcDNA group,the number of cell clones,scratch-healing rate,and number of invasive cells were higher in the UM+miR-545-3p-mimics+E2F3 group,expression levels of E2F3,CDK1,MMP-2,MMP-9,N-Cadherin,and Vimentin were higher,and expression levels of miR-545-3p and E-Cadherin were lower(P<0.05).Tumor volume and mass were smaller in the miR-545-3p-mimics group compared with the miR-NC group,while E2F3 expression levels were lower and miR-545-3p levels were higher(P<0.05).Tumor volume and mass were smaller in the UM+miR-545-3p-mimics group compared with the miR-545-3p-mimics group,E2F3 expression levels were lower and miR-545-3p levels were higher(P<0.05).Conclusions UM-mediated miR-545-3p inhibits the proliferation,migration,invasion,and EMT of PTC cells by targeting the E2F3 signaling pathway.