两种复合侵染辣椒的马铃薯Y病毒属病毒分子鉴定OA

To clarify the molecular characteristics and host range of Yuxi,Yunnan isolates of Chili vein mottle virus(ChiVMV)and Chili ringspot virus(ChiRSV),and to provide a scientific basis for the precise prevention and control of pepper viral diseases in this region,this study used pepper samples suspected of coinfection collected from Yuxi,Yunnan as materials.The isolates ChiVMV-LJ6 and ChiRSV-LJ6 were obtained by RT-PCR amplification.DNAMAN 8 and MEGA-X software were used for whole-genome nucleotide identity analysis and phylogenetic tree construction of the two isolates,and the infectivity of these two viruses to 7 pepper varieties was explored by friction inoculation.The results showed that after removing the poly(A)tail,the full-length genome of ChiVMV-LJ6 was 9 718 bp,and that of ChiRSV-LJ6 was 9 652 bp.Phylogenetic analysis indicated that ChiVMV-LJ6 had the closest evolutionary relationship with the Yunnan pepper ChiVMV isolate(GenBank accession no.ON 168943.1),while ChiRSV-LJ6 was most closely related to the Yunnan pepper ChiRSV isolates(GenBank accession nos.KX258620.1 and MZ269480.1).All 7 tested pepper varieties could be infected by the two viruses either individually or in combination,and there were significant differences in symptoms among different varieties after infection.This study clarified the molecular characteristics and wide host range of ChiVMV-LJ6 and ChiRSV-LJ6,and provided an important theoretical basis for the precise prevention and control of pepper virus coinfection and the breeding of disease-resistant varieties in this region.