NaCl胁迫下橙色大白菜AP2-ERF转录因子表达分析及基因克隆OA

To identify genes of the AP2-ERF transcription factor family with altered expression levels in orange Chinese cabbage leaves under NaCl stress,orange Chinese cabbage seedlings were subjected to salt stress treatments with 0,150,and 300 mmol·L-1 NaCl solutions.Key AP2-ERF family genes responding to NaCl stress were screened via RNA-seq,and their differential expression trends were validated using quantitative real-time polymerase chain reaction(qRT-PCR).Subsequently,the full-length RAP2.2 gene was cloned by RT-PCR,followed by bioinformatics analysis of this gene.The results showed that under 0-300 mmol·L-1 NaCl stress:the expression levels of AP2-ERF,RAP2.2,RAP2.7,RAP2.12,TOE2,and Atlg16060-like(homologous to Arabidopsis thaliana Atlg16060)in seedling leaves first increased and then decreased,peaking under the 150 mmol·L-1 NaCl treatment;the expression levels of RAP2.1,RAP2.3,RAP2.4,RAP2.11,and At2g41710-like(homologous to Arabidopsis thaliana At2g41710)generally showed an upward trend,reaching the peak under the 300 mmol·L-1 NaCl treatment;RAV1 was down-regulated;while the expression level of PLT2 showed no significant difference.The full-length cDNA of the cloned RAP2.2 gene was 1 307 bp,encoding 352 amino acids.This study clarified the expression characteristics of AP2-ERF family genes and the molecular properties of RAP2.2 in orange Chinese cabbage under salt stress,providing a theoretical basis and genetic resources for subsequent analysis of the salt stress response mechanism mediated by this gene family and salt-tolerant molecular breeding of orange Chinese cabbage.