海南产对叶百部中1个新的降倍半萜OA

Objective To investigate the chemical components and anti-inflammatory activities of the roots of Stemona tuberosa from Hainan province.Methods The chemical constituents of S.tuberosa were isolated and purified using chromatography techniques such as silica gel,gel column chromatography,and semi-preparative high-performance liquid chromatography(HPLC).Subsequently,the structures of the purified compounds were identified by modern spectroscopic methods such as nuclear magnetic resonance(NMR)and mass spectrometry(MS).Finally,the anti-inflammatory activities of the compounds were evaluated using the lipopolysaccharide(LPS)-induced murine monocyte-macrophage RAW264.7 cell model.Results A total of seven compounds were isolated from S.tuberosa,and identified as(4R)-3,5,5-trimethyl-4-(3-methylbut-2-enoyl)cyclohex-2-en-1-one(1),oxystemofoline(2),methyl 4-hydroxycinnamate(3),(-)-stemoamide(4),tuberostemospiroline(5),stemanthrene C(6),stilbostemin F(7).Compounds 2 and 3 exhibited inhibitory activity against NO production in LPS-induced RAW264.7 cells,with median inhibition concentration(IC50)values of(42.99±4.96)and(70.22±0.81)μmol/L,respectively.Conclusion Compound 1 was identified as a new compound,named tuberostemoterpenone.Compound 2 was isolated from S.tuberosa for the first time.Compounds 2 and 3 exhibited no significant cytotoxicity within the tested concentration range and demonstrated certain anti-inflammatory activity.