反柄紫芝化学成分及其生物活性研究OA

Objective To investigate the chemical constituents from the methanol extract of the polyporaceae fungus Ganoderma cochlear and preliminarily evaluate their anti-atherosclerotic activity in vitro.Methods The extract was systematically isolated and purified by D-101 macroporous adsorption resin,silica gel column chromatography,reversed-phase C18 column chromatography,preparative thin layer chromatography and semi-preparative HPLC.The structures of the isolated compounds were elucidated by HR-ESI-MS,IR,and NMR.Meanwhile,the absolute configurations of the new compounds were determined by NMR and ECD calculation.The in vitro anti-atherosclerotic activities of the isolates were assessed using an oxidized low-density lipoprotein(ox-LDL)-induced macrophage lipid deposition model.Results Two novel compounds were isolated and identified from G.cochlear,designated as 7,10-epoxy-3,4-seco-9(10→19)abeo-25,26,27-trinorlanosta-4,11-dioxo-8-en-3,24-dimethyl ester(1)and 6-hydroxy-2-(4-methylpent-3-en-1-yl)-4H-chromen-4-one(2).In the ox-LDL-induced macrophage model,compounds 1 and 2 did not exhibit significant anti-lipid deposition activity at 40 μmol/L.Conclusion This study successfully isolated and identified two new compounds,and named as ganodeconoid J(1)and ganodeconoid K(2).Compound 1 represents the first Ganoderma triterpenoid possessing a 5β-H,enriching the chemical diversity of Ganoderma fungi.Compounds 1 and 2 did not show significant in vitro anti-atherosclerotic activity.