高铜负荷对大鼠神经行为和SIRT3/PGC-1α信号通路的影响OA

Objective To investigate the influence of high copper load on neurobehavior in rats and its possible mechanism of action based on the deacetylase sirtuin 3(SIRT3)/peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α)signaling pathway.Methods A total of 50 adult Sprague-Dawley rats were randomly divided into model group and blank group,with 25 rats in each group.The rats in the model group were fed with 1 g/kg high-copper diet and 0.185%copper sulfate solution(prepared using deionized water)to establish a rat model of high copper load,and after 12 weeks,the shuttle box test and the pole-climbing test were used to observe neurobehavioral changes,while Western blotting and immunofluorescent staining were used to measure the protein expression levels of SIRT3 and PGC-1α in hippo-campal tissue.Results The shuttle box test showed that compared with the blank group,the model group had a signifi-cant reduction in the number of times of active avoidance reaction after stimulation(P<0.01)and significant increases in the times of active avoidance reaction and passive avoidance reaction(P<0.01);the pole-climbing test showed that com-pared with the blank group,the model group had a significant increase in pole-climbing time(P<0.01).Western blotting and immunofluorescent staining showed that compared with the blank group,the model group had significant reductions in the protein expression levels of SIRT3 and PGC-1α in hippocampal tissue(P<0.05).Conclusion High copper load has a marked influence on the neurobehavior of rats,possibly by regulating the expression of the SIRT3/PGC-1α signal path-way in hippocampal neurons.