基于液相色谱-串联质谱技术的阿胶粉蛋白组分分析及其抗氧化活性评价OA
Proteomic Analysis and the Antioxidant Activity Evaluation of Asini Corii Colla Powder Based on Liquid Chromatography-Tandem Mass Spectrometry Technology
阿胶中含有多种具有生物活性的蛋白质,这些成分使阿胶在保健方面展现出多重功效.本研究运用液相色谱-串联质谱(LC-MS/MS)方法,对阿胶粉中的蛋白质组成进行了系统鉴定与分析,鉴定出多种功能蛋白,包括多种中间纤维蛋白、胶原蛋白、免疫调节蛋白以及多种抗氧化相关蛋白.进一步通过使用人真皮成纤维细胞(HSF)评估阿胶粉的细胞毒性、增殖及迁移能力.结果表明,阿胶粉在实验质量浓度范围内(0.156～20 mg/mL)无显著细胞毒性,并能促进HSF细胞增殖和迁移,初步表明了阿胶粉在皮肤修复领域的潜力.为探究阿胶的抗氧化应激活性,进一步采用过氧化氢(H2O2)诱导细胞氧化应激模型,检测阿胶粉对细胞内活性氧(ROS)的清除能力及抗氧化酶活性的影响.实验表明,经阿胶粉预处理后,H2O2诱导的ROS水平被显著抑制,同时细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH～Px)的活性均有所提升,这证实其抗氧化作用与增强内源性抗氧化防御系统有关.蛋白质组学分析进一步揭示,阿胶粉可能通过调控热休克蛋白B家族、胆绿素还原酶B、血红蛋白亚基α以及核因子κB(NF-KB)信号通路相关蛋白的表达,调控机体氧化应激,在一定程度上减轻细胞氧化应激损伤.综上,本研究阐明了阿胶的蛋白质组成及验证其在抗氧化应激中的作用与机制,这为拓展阿胶在皮肤修复与抗衰老领域的应用奠定了科学基础.
Asini corii colla contains multiple bioactive proteins,endowing it with multifaceted health benefits.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)to systematically identify and analyze the protein composition of Asini corii colla powder,identifying various functional proteins including multiple intermediate filament proteins,collagens,immunomodulatory proteins,and antioxidant-related proteins.Further evaluation of Asini corii colla powder's cytotoxicity,proliferation,and migration effects was conducted using human dermal fibroblasts(HSF).Results indicated no significant cytotoxicity within the tested concentration range(0.156～20 mg/mL),while promoting HSF proliferation and migration,suggesting preliminary potential for Asini corii colla powder in skin repair applications.To investigate the antioxidant stress activity of Asini corii colla,this study further employed a hydrogen peroxide(H2O2)induced cellular oxidative stress model to evaluate the effects of Asini corii colla powder on intracellular reactive oxygen species(ROS)scavenging capacity and antioxidant enzyme activity.Experiments demonstrated that pre-treatment with Asini corii colla powder significantly suppressed H2O2-induced ROS levels while enhancing intracellular superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.This confirms its antioxidant effects are associated with strengthening the endogenous antioxidant defense system.Proteomics analysis further revealed that Asini corii colla powder may regulate oxidative stress by modulating the expression of proteins associated with the heat shock protein B family,biliverdin reductase B,hemoglobin subunit a,and nuclear factor KB(NF-KB)signaling pathways,thereby mitigating cellular oxidative stress damage to a certain extent.In summary,this study elucidates the protein composition of Asini corii colla and validates its role and mechanisms in combating oxidative stress,laying a scientific foundation for expanding its applications in skin repair and anti-aging.
解晓;李聪;张建岭;陈智杰;陶春香;扶美梅;尤惠琳;郭金山
东阿阿胶健康产业科技有限公司,聊城市功能性食品研究开发重点实验室,营养健康消费品聊城市工程研究中心,聊城 252201东阿阿胶健康产业科技有限公司,聊城市功能性食品研究开发重点实验室,营养健康消费品聊城市工程研究中心,聊城 252201东阿阿胶健康产业科技有限公司,聊城市功能性食品研究开发重点实验室,营养健康消费品聊城市工程研究中心,聊城 252201东阿阿胶健康产业科技有限公司,聊城市功能性食品研究开发重点实验室,营养健康消费品聊城市工程研究中心,聊城 252201中国科学院长春应用化学研究所,中国科学院高性能合成橡胶及其复合材料重点实验室,长春 130022南方医科大学基础医学院组织与胚胎学教研室,国家药品监督管理局化妆品安全评价重点实验室,广东省药品监督管理局化妆品安全与功效关键技术重点实验室,广州 510515广东粤港澳大湾区黄埔材料研究院,再生医学与修复材料中心,广州 511363中国科学院长春应用化学研究所,中国科学院高性能合成橡胶及其复合材料重点实验室,长春 130022||南方医科大学基础医学院组织与胚胎学教研室,国家药品监督管理局化妆品安全评价重点实验室,广东省药品监督管理局化妆品安全与功效关键技术重点实验室,广州 510515||广东粤港澳大湾区黄埔材料研究院,再生医学与修复材料中心,广州 511363
化学化工
阿胶粉蛋白质组学活性氧清除氧化应激
Asini corii collaProteomicsReactive oxygen species scavengeOxidative stress
《应用化学》 2026 (3)
396-410,15
东阿阿胶健康产业科技有限公司横向项目(No.DEEJ-YB-2024-10-190)资助 Supported by the Industry-sponsored Project with Dong'e Ejiao Health Products Co.,Ltd.(No.DEEJ-YB-2024-10-190)
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