汉氏葡糖醋杆菌HDM1-3细菌纤维素合成的代谢组学研究OA

To investigate the metabolic shift mechanism of Komagataeibacter hansenii(K.hansenii)HDM1-3 from the bacterial growth phase to the bacterial cellulose(BC)synthesis phase,untargeted metabolomics analysis was performed on K.hansenii HDM1-3 cultures at the early fermentation stage(18 h)and late fermentation stage(36 h).Metabolite differential screening identified 211 differential metabolites,with 92 metabolites upregulated and 119 metabolites downregulated at 36 h compared to 18 h.Pathway enrichment analysis indicated that these metabolites were mainly associated with five key metabolic pathways:starch and sucrose metabolism,galactose metabolism,glycerophospholipid metabolism,amino sugar and nucleotide sugar metabolism,and sphingolipid metabolism.Further screening of differential metabolites using the KEGG database showed that UDP-glucose,ADP-glucose,melibiose,and sphinganine exhibited significant abundance differences between 18 h and 36 h of fermentation,suggesting their potential as biomarkers for different fermentation stages.In summary,this study analyzed the metabolic differences in K.hansenii HDM1-3 before and after BC biosynthesis,proposed a potential response strategy of this strain to acid stress at the metabolic level,provided key data for elucidating the metabolic regulatory mechanism underlying the transition from the growth phase to cellulose synthesis in K.hansenii HDM1-3,and identified potential metabolic intervention targets for the subsequent targeted optimization of BC production.