天麻极细粉重复给药毒性试验研究OA

This study aimed to investigate the toxic effects of repeated administration of ultrafine powder of Gastrodia elata in Sprague-Dawley(SD)rats and to provide a scientific basis for its safety evaluation in medicinal use.A total of 128 SD rats were randomly divided into a negative control group and low,medium,and high-dose groups of Gastrodia elata ultrafine powder(0.7,1.4,and 2.8 g/kg),with 32 rats in each group and an equal number of males and females.All rats were administered the test substance continuously for 26 weeks,followed by a 4-week recovery period after the end of administration.The general condition of the rats was observed daily,and body weight and food intake were measured weekly.At the end of the dosing period and the recovery period,necropsy was performed,and hematological parameters,serum biochemical indices,electrolytes,coagulation function,and major organ coefficients were determined.Tissue morphology was examined both macroscopically and microscopically.Compared with the negative control group,no toxicological changes related to the test substance were observed in any of the measured parameters in either male or female rats at all dose levels.Under the conditions of this study,2.8 g/kg was identified as the no-observed-adverse-effect level(NOAEL)for Gastrodia elata ultrafine powder.However,during long-term high-dose consumption in daily use,particular attention should be paid to changes in appetite and body weight.