药用植物雀舌木属叶绿体基因组的比较研究OA

To clarify the characteristics of Leptopus Decne.sequences,codon usage patterns,influencing factors,and phylogeny,we perform a comparative analysis of the chloroplast genomes of Leptopus chinensis and L.cordifolius with medicinal value.The whole genome of L.chinensis chloroplasts is sequenced,assembled,and annotated by second-generation high-throughput sequencing technology,and further analyzes its genome structure,repeat sequences,codon usage preference,and phylogeny.The chloroplast genome of Leptopus exhibited a typical double-stranded circular tetrad.The IR region reflects high conservatism,and there are more mutation sites in the LSC and SSC regions,while the LSC region contains more SSRs.Codon usage analysis indicated a non-neutral,with a preference for using A/U at the third codon position.The phylogenetic tree constructed based on the whole genome of chloroplasts revealed a close phylogenetic relationship with 100％suppor.In conclusion,selection pressure has a great influence on the codon preference of the chloroplast genome of Leptopus,and thess results can provide genetic data support for futher studies on the metabolism and utilization of natural components of Leptopus.