介孔碳负载改性叶绿素Ce6光动力治疗肿瘤机制的研究OA

Spherical mesoporous Fe-N-DNC was synthesized using dopamine hydrochloride as the carbon precursor via a soft-templated strategy,with Fe3O4 nanoparticles confined in the mesoporous carbon framework.Chlorin e6(Ce6)was subsequently loaded onto the Fe-N-DNC surface through π–π interactions,followed by polyethylene glycol(PEG)modification to obtain PEG@Ce6@Fe-N-DNC nanomaterials.The morphology,structural features,and chemical composition of the as-prepared nanomaterials were systematically characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and Raman spectroscopy,together with an evaluation of singlet oxygen generation capability.The results demonstrate that PEG@Ce6@Fe-N-DNC exhibits a uniform spherical morphology with an average particle size of approximately 200 nm and favorable photodynamic activity.The photodynamic therapeutic performance of PEG@Ce6@Fe-N-DNC was further evaluated in A431 cutaneous squamous cell carcinoma cells under 660 nm light irradiation.The results show that PEG@Ce6@Fe-N-DNC induces significant cytotoxicity upon light activation,with an IC₅₀ value of 10 μg/mL.These findings indicate that PEG@Ce6@Fe-N-DNC nanomaterials exhibit effective single-modal photodynamic antitumor activity and provide experimental evidence for the application of Ce6-based nanomaterials in the photodynamic treatment of cutaneous squamous cell carcinoma.