肺癌ALK(Ventana/D5F3)非特异性表达5例及其临床特征分析OA

Objective To investigate the expression of anaplastic lymphoma kinase(ALK,Ventana/D5F3)in five cases of non-adenocarcinoma lung cancer and analyze their related clinicopathological features.Methods A total of five specimens from patients with non-adenocarcinoma lung cancer who were examined and diagnosed in the Department of Pathology of The First People's Hospital of Yulin form December 2023 to December 2024 were collected.Among them,there were three cases of small cell carcinoma,one case of squa-mous cell carcinoma,and one case of small cell carcinoma transformation after squamous cell carcinoma treat-ment.Immunohistochemistry was performed to detect ALK protein,and fluorescence in situ hybridization(FISH)was used for verification.Results Among the five lung cancer patients,four were male and one was female,aged from 52 to 82 years with a median age of 67 years.Squamous cell carcinoma tumor cells were round or oval,with an increased nuclear-cytoplasmic ratio,abundant and lightly stained cytoplasm,and visible mitotic figures.Microscopic observation of small cell carcinoma showed that the tumor cells were irregular,with salt-and-pepper nuclei,scarce cytoplasm,some nuclei were deformed by compression,and mitotic figures were easily seen.The results of ALK(Ventana/D5F3)immunohistochemistry showed strong positive expres-sion in the cytoplasm(the positive proportion ranging from 5％to 40％),while no ALK fusion gene was de-tected by FISH.Conclusion ALK(Ventana/D5F3)is predominantly expressed in pulmonary non-small cell lung cancer,mainly adenocarcinoma,but may have nonspecific expression in other types of tumors.