乳腺癌组织中QPRT和SHMT2的表达及其临床意义OA

Objective To investigate the expressions of quinolinate phosphoribosyltransferase(QPRT)and serine hydroxymethyltrans-ferase 2(SHMT2)in breast cancer tissues and their correlation with the clinicopathological characteristics and prognosis of the patients.Methods A total of 236 cases of non-special type breast carcinoma and 118 matched adjacent normal breast tissues archived in Yunnan Cancer Hospital from January 2020 to June 2023 were enrolled in this study.The expressions of QPRT and SHMT2 proteins in tissues were detected by immunohistochemistry.The Kaplan-Meier plotter database was used to analyze the impact of QPRT and SHMT2 expressions in breast cancer tissues on the prognosis of the patients.The Gene Expression Profiling Interactive Analysis(GEPIA)database was utilized to analyze the mRNA expressions of QPRT and SHMT2 in breast cancer tissues and their correlation.Results The high expression rates of QPRT and SHMT2 in breast cancer tissues were significantly higher than those in adjacent tissues(QPRT:25.0%vs 5.1%,SHMT2:66.5%vs 51.7%,both P<0.01).The expression of QPRT protein in breast cancer tissues was significantly different in terms of TNM stage,histological grade,and lymph node metastasis(all P<0.05),while the expression of SHMT2 protein was significantly different in terms of TNM stage and lymph node metastasis(both P<0.05).QPRT expression in human epidermal growth factor receptor 2(HER2)-overexpressing breast cancer tissues was higher than those in luminal A,luminal B1,and triple-negative breast cancer tissues(all P<0.05).No significant difference in SHMT2 expression was observed among different breast cancer molecular subtypes(P>0.05).The Kaplan-Meier plotter database analysis revealed that breast cancer patients with either low QPRT or low SHMT2 expression exhibited superior overall survival,distant metasta-sis free survival,and recurrence-free survival compared to those with high expression of QPRT or SHMT2(all P<0.05).Furthermore,low SHMT2 expression was also associated with improved post-progression survival(P<0.05).The GEPIA database analysis showed that there was a positive correlation between QPRT and SHMT2 protein expressions in breast cancer tissues(r=0.23,P<0.01).Conclusions The high expressions of QPRT and SHMT2 is related to the occurrence,development,and poor prognosis of breast cancer.The expression of QPRT is related to different types of breast cancer.The expressions of QPRT and SHMT2 are positively correlated.