慢性胃炎湿证患者舌苔菌群特征研究OA

Objective To explore the correlation between tongue coating microbiota and traditional Chinese medicine(TCM)syndromes by analyzing the characteristics of tongue coating microbiota in chronic gastritis patients with and without dampness syndromes,thereby providing data support for the objectification and biological research of dampness syndromes.Methods A total of 328 chronic gastritis patients were enrolled and divided into the dampness syndrome group(n=153)and non-dampness syndrome group(n=175)according to TCM syndrome differentiation criteria.The dampness syndrome group was further subdivided into the cold-dampness affecting the spleen(CDAS)subgroup(n=70)and damp-heat in the spleen-stomach(DHSS)subgroup(n=83),while the non-dampness syndrome group included the deficiency-cold of spleen-stomach(DCSS)subgroup(n=98)and stomach-yin deficiency(SYD)subgroup(n=77).Additionally,50 healthy volunteers were recruited as the healthy control(HC)group.High-throughput 16S rDNA sequencing technology was used to detect and analyze the abundance and diversity of tongue coating microbiota in each group.Spearman correlation analysis was used to investigate the relationship between tongue coating microbiota and TCM syndromes.Results ①Differential bacterial genera analysis:Compared with the HC group,18 bacterial genera showed significant differences in abundance in the CDAS subgroup(P<0.05,LDA>0.7),and also 18 bacterial genera exhibited significant differences in the DHSS subgroup(P<0.05,LDA>0.7).Among these differential genera,17 were shared between the two subgroups.Of these shared genera,7 showed significantly decreased(P<0.05,LDA>0.7)abundance and 10 showed significantly increased(P<0.05,LDA>0.7)abundance in the HC group.Compared with the DCSS subgroup,4 bacterial genera displayed significant differences in the CDAS subgroup(P<0.05,LDA>0.7).At the phylum level,1 phylum showed a significant difference between the CDAS subgroup and the DHSS subgroup(P<0.05,LDA>0.7).②Correlation analysis:DHSS syndrome was significantly correlated with 7 bacterial genera:it was positively correlated with Selenomonas(R=0.21,P<0.001),Megasphaera(R=0.34,P<0.001),Atopobium(R=0.28,P<0.001),and negatively correlated with Fusobacterium(R=-0.21,P<0.001),Catonella(R=-0.24,P<0.001),Gemella(R=-0.29,P<0.001),Haemophilus(R=-0.28,P<0.001).DCSS syndrome was significantly correlated with 2 bacterial genera:it was positively correlated with Peptostreptococcus(R=0.25,P<0.001),Catonella(R=0.23,P<0.001).Conclusions Significant differences in tongue coating microbiota composition exist at both the phylum and genus levels among chronic gastritis patients with dampness syndromes(CDAS,and DHSS),patients with non-dampness syndromes(DCSS,and SYD)and healthy individuals.Moreover,DHSS syndrome and CDAS syndrome correlate with specific bacterial genera.These findings suggest that the characteristics of the tongue coating microbial community may serve as objective biomarkers for dampness syndromes in chronic gastritis,providing novel microbiological evidence for the objectification research of TCM dampness syndromes.