基于磁共振波谱测温的C6胶质瘤大鼠脑温度梯度研究OA

Objective:To investigate the spatial distribution characteristics of brain temperature in a rat C6 glioma model and its value in defining the tumor infiltration boundary.Methods:Sixteen Sprague-Dawley(SD)rats were randomly divided into a glioma group(n=8)and a control group(n=8).The glioma group underwent stereotactic injection of C6 glioma cells into the right caudate nucleus,while the control group received an equal volume of culture medium.On the 14th day post-injection,all rats underwent conventional MRI and proton magnetic resonance spectroscopy(1H-MRS)on a 7T MRI scanner.Spectra were acquired from the tumor solid part,peritumoral area,and contralateral normal caudate nucleus(symmetric bilateral locations for the control group).Absolute brain temperature for each volume of interest(VOI)was calculated using an in vitro temperature calibration model.Results:In glioma-bearing rats,brain temperature differed significantly among the various brain regions(F=37.05,P<0.01).The temperature was highest in the tumor solid part(38.53±0.24℃),which was significantly higher than that in the peritumoral area(38.14±0.18℃,P<0.01)and the contralateral normal brain area(37.63±0.19℃,P<0.01).The temperature in the peritumoral area was also significantly higher than that in the contralateral normal brain area(P<0.01).In the control group,there was no significant temperature difference between the bilateral brain regions(P>0.05).The temperature in the contralateral normal brain area of the glioma group was significantly higher than that in the corresponding brain area of the control group(P<0.01).Conclusion:MRS thermometry can sensitively detect brain temperature gradients in the C6 glioma model.The elevated temperature in the peritumoral area provides a potential biomarker for identifying the tumor infiltration boundary.