盔形溞头盔发育相关基因的功能验证:基于RNA干扰与形态观测分析OA

In order to explore the molecular mechanism of helmet development in Daphnia galeata,this study investi-gated the expression of two genes related to helmet development(DgTrepsin and DgFerritin)and changes of helmet morphology in D.galeata under RNA interference.The results showed that at a 2%concentration of E.coli,compared with the EGFP group,the DgTrypsin-interference group exhibited significant reductions in helmet length,relative helmet length,and the ratio of helmet length to head width,accompanied by a 27.45%decrease in DgDrypsin expres-sion.However,no significant morphological or gene expression changes were observed in the DgFerritin-interference group at this concentration.When the concentration concentration of E.coli was increased to 5%,both the DgTrypsin and DgFerritin groups showed significant decreases in all three helmet morphology metrics,along with respective gene expression reductions of 55.96%and 40.39%,respectively.These results indicate that higher levels of RNA interfe-rence have a stronger inhibitory effect on the morphological changes and expression levels of the target genes.The findings demonstrate that DgTrypsin and DgFerritin genes are involved in the development of D.galeata helmet providing a valuable reference for further exploration of molecular mechanisms underlying the morphogenesis in Daph-nia.