复合菌群对厌氧消化强化效果及沼气产量影响因素分析OA

[Objective]To obtain an efficient compound microbial consortium,the effects of different bioaugmentation treatments on biogas yield in food waste anaerobic digestion(AD)were investigated to elucidate the mechanisms by which the compound microbial consortium enhances CH4 yield.[Method]Four treatments were set up for the sequential batch AD experiment:addition of sterile water(CK),inoculation with Methanosarcina barkeri(B),inoculation with enriched methanogenic community(J),and inoculation with constructed microbial consortium comprising M.barkeri and methanogenic community in a 1∶1 based on biomass(BJ).The characteristics of biogas production were analyzed using gas chromatography and the modified Gompertz model.Additionally,a random forest model was utilized to identify the key factors influencing biogas yield.[Result]The results showed that all bioaugmentation treatments extended the biogas production period and significantly enhanced the biogas production rate,cumulative biogas yield,and CH4 content(P<0.05).Notably,the BJ group showed the most significant improvement.BJ group presented the highest methanogenic potential,followed by J and B groups.Temperature,substrate TS content,pH value,trace elements(TE)concentration,substrate C/N ratio(P<0.01),and inoculation amount of the compound microbial consortium all had significant effects on biogas yield(P<0.05),with temperature exerting the most substantial impact.The locally weighted regression(LOESS)model showed a nonlinear relationship between each factor and biogas yield,with each factor having its optimal range.[Conclusion]The compound microbial consortium integrated the advantages of the methanogenic community and M.barkeri,balancing functional complexity and stability.This effectively enhanced upstream metabolic processes and methanogenesis,significantly increasing biogas yield and CH4 content in the AD system.By optimizing the bioaugmentation combination and regulating key process parameters,the performance of AD can be effectively improved,promoting waste resource utilization and the sustainable development of energy.