抗菌肽对哺乳期犊牛生长性能、腹泻指数、血清指标及粪便微生物群落的影响OA

The experiment aimed to investigate the effects of antimicrobial peptide on the growth performance,diarrhea index,serum indexes,and fecal microbiota of suckling calves.A total of 60 healthy and similarly weighted one-day-old Holstein calves were randomly divided into four groups,with three replicates per group,and five calves per replicate.The control group did not add antimicrobial peptide,while the groups Ⅰ,Ⅱ,Ⅲ added 2,4,and 8 g/(head·d)antimicrobial peptide to the milk replacer powder,respectively.The pre-test period was eight days,and the formal test period was 22 days.The results showed that there was no significant difference in growth performance among the groups(P>0.05).The duration of diarrhea,number of diarrhea calves,diarrhea index,and medication cost in the experimental groups were lower than those in the control group,and the fecal score was significantly higher than that in the control group(P<0.05).The levels of interleukin-4(IL-4),immunoglobulin A(IgA),and immunoglobulin G(IgG)in the serum of group Ⅰ were significantly higher than those in other groups(P<0.05).The activity of glutathione peroxidase(GSH-Px)in groups Ⅰand Ⅱ was significantly higher than that in the control group(P<0.05).The relative abundance of Actinobacteria in the feces of group Ⅲ calve was higher than that of other groups,while the relative abundance of Bacteroidetes in groups Ⅱand Ⅲ was higher than that of group Ⅰ and the control group.The relative abundance of Bacteroides in group Ⅲ was higher than that in the control group,and the Chao1 index was significantly higher than that in the control group(P<0.05).The study shows that the antimicrobial peptide can enhance the immune function and antioxidant capacity,regulate the intestinal microbial community structure of suckling calves.The optimal amount of antimicrobial peptide added is 8 g/(head·d).