苔藓植物组织培养与应用研究进展OA

Bryophytes are the most primitive terrestrial group among higher plants,existing in the transitional environment from aquatic to terrestrial,and they have significant ecological functions.Moreover,bryophytes are rich in diverse secondary metabolites with biological activities,showing broad application prospects in fields such as medicine.However,wild bryophytes are difficult to identify and enrich due to their small size and complex communities,which greatly limits in-depth research and development and utilization of bryophytes.Therefore,establishing an efficient tissue culture technology system for bryophytes is of great theoretical and practical significance.This paper reviews the key influencing factors of bryophyte tissue culture,focuses on discussing core technical links such as explant selection and disinfection,and culture medium optimization,and systematically summarizes the applications of tissue-cultured bryophytes in multiple fields,including as indicators of environmental pollution and materials for environmental remediation,production of secondary metabolites with medicinal value,as bioreactors and genetic transformation materials for producing complex proteins or drugs,and screening of stress-resistant genes to enhance plant adaptability to extreme environments.It also analyzes the existing problems in bryophyte tissue culture and proposes future research directions,providing important references for the basic research and practical application of bryophytes.