生姜转录因子ZoERF35基因克隆与特征分析OA

[Objectives]This study aimed to explore the structural characteristics and protein properties of the ZoERF35 transcription factor gene,which was significantly induced by high temperature stress,laying the groundwork for revealing the molecular regulatory mechanisms of ZoERF35 in ginger's response to high temperature stress.[Methods]Using'Zhugen ginger'as the material,ZoERF35 transcription factor gene was cloned from the ginger leaves.The characteristics were analyzed through bioinformatics,subcellular localization,and transcriptional self-activation analysis,and its expression patterns were examined using RT-qPCR.[Results]ZoERF35 encoded a total of 214 amino acids,belonging to a hydrophilic and unstable protein.This protein lacked transmembrane structure regions but contained 36 potential phosphorylation sites.Phylogenetic tree analysis revealed that the ginger ZoERF35 protein was most closely related to KAG1362132.1 protein from Cocos nucifera,both possessing a highly conserved AP2 domain.Additionally,the ZoERF35 protein might interact with transcription factors such as ERF5,ERF104,WRKY33 and WRKY40,collectively participating in the abiotic stress response.Subcellular localization and yeast two-hybrid assays results showed that ZoERF35 was localized in the nucleus and had no transcriptional self-activation activity.After high-temperature treatment,the relative expression level of ZoERF35 in ginger roots,stems,and leaves increased by 18.04,33.16,and 1.12 times,respectively.[Conclusions]The ZoERF35 transcription factor in ginger was significantly induced under high-temperature stress.The gene contained multiple stress-related cis-acting elements,and interacted with other transcription factors,collectively participating in the abiotic stress response.