少腹逐瘀丸对子宫内膜异位症大鼠MMP-9及TIMP-2表达的影响OA

Aim To analyze the effects of Shaofu Zhuyu Pills on the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-2(TIMP-2)in rats with endometriosis(EMs).Methods Female rats were randomly divided into a sham-operated group,a model group,a low-dose group(2.8 g/kg Shaofu Zhuyu Pills),a high-dose group(5.6 g/kg Shaofu Zhuyu Pills),and a gestrinone group(1.25 mg/kg gestrinone).EMs models were established in all groups except the sham-operated group.Serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,estradiol(E2),progesterone(P),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were compared.Pathological changes were observed by HE staining,and the mRNA and protein expression levels of MMP-9 and TIMP-2 in endometrial tissue were compared.Results Compared with the sham-operated group,the EMs model group showed significant pathological damage in the endometrial tissue.However,intervention with Shaofu Zhuyu Pills significantly improved this pathological damage(P<0.05).The mass and volume of ectopic lesions in the high-dose group and the gestrinone group were lower than those in the model group(P<0.05).Compared with the sham-operated group,the model,low-dose,high-dose,and gestrinone groups showed increased serum levels of TNF-α,IL-6,IL-1β,E2,P,and elevated mRNA and protein expression of MMP-9,while levels of FSH,LH,and mRNA and protein expression of TIMP-2 were decreased(P<0.05).Compared with the model group,these trends were reversed in the high-dose and gestrinone groups(P<0.05).Conclusion Shaofu Zhuyu Pill intervention in EMs rats can significantly improve pathological damage in en-dometrial tissue,alleviate inflammatory responses,and normalize sex hormone levels.Its mechanism of action may involve the down-regulation of MMP-9 expression and upregulation of TIMP-2 expression.