丰富环境上调m6A修饰促进小鼠视皮质突触可塑性相关基因蛋白的表达OA

Aim To investigate the effects of enriched environment(EE)on m6A modification and the expression of synap-tic plasticity-related genes and proteins in the mouse visual cortex.Methods Fourteen 8-week-old male C57BL/6J mice were randomly divided into a control group(CON group)and an EE group.Dot blot hybridization and immunohistochemistry were used to detect m6A modification levels in the visual cortex.Western blotting was performed to analyze the expression of methyltransferases and demethylases.qRT-PCR was used to measure mRNA levels of synaptic protein-encoding genes,and Western blotting was applied to detect synaptic protein expression.Results Compared with the CON group,the EE group showed increased m6A modification levels and downregulated expression of the m6A demethylase ALKBH5(P<0.05),while no significant differences were observed in other methylation-related enzymes(P>0.05).Although no significant differences were found in the mRNA levels of synaptic protein genes SYP,PSD-95,and Homer-1 between the two groups(P>0.05),the protein levels of SYP,PSD-95,and Homer-1 were significantly up-regulated in the EE group(P<0.05).Conclusion Enriched environment upregulates m6A modification of plasticity-related genes in the mouse visual cortex,thereby promoting their protein translation and leading to increased expression of synaptic proteins.