青海省甘蒙柽柳根际土壤细菌群落特征及功能分析OA

[Objective]This study systematically analyzed the diversity,community composition,and growth-promoting functions of rhizosphere soil bacteria in Tamarix austromongolica from Qinghai Province.The research aims to provide a basis for the conservation of this tree species and the development of its indigenous microbial resources.[Method]Using the rhizosphere soil of T.austromongolica in three conservation areas,Ranguo Village(RG),Shanglujuan Village(SL),and Shangjiawugou Village(SJ)in Hainan Zang Autonomous Prefecture,Qinghai Province as the research object,the bacterial diversity and community composition of the rhizosphere soil of T.austromongolica were analyzed based on high-throughput sequencing,rhizosphere soil bacteria were isolated using the traditional dilution culture method,and functional evaluation was performed with functional plates and specific color changes.[Result]There are significant differences in the Alpha and Beta diversity of rhizosphere soil bacteria in T.austromongolica from Ranguo Village(RG)compared to Shanglujuan Village(SL)and Shangjiawugou Village(SJ).However,the dominant bacterial phyla across all three conservation areas are Proteobacteria,Acidobacteriota,and Actinomycetota.Pseudomonas,unclassified_bacteria,and unclassified_Gemmatimonadaceae are the biomarker for the different conservation areas,respectively.A total of 55 strains are isolated from the rhizosphere soil of T.austromongolica across different conservation areas,among which two strains are potential new species,and 11 strains belong to the core genera of Streptomyces,Sphingomonas,Lysobacter and Devosia.Twenty-seven strains exhibit potential growth-promoting functions,with 12 strains processing inorganic phosphorus-dissolving ability,eight strains demonstrating organic phosphorus-mineralizing ability,eight strains showing potassium-dissolving ability,and 15 strains capable of IAA-producing ability.Among there,the strain Oceanbacillus sojae RGGN1 isolated from RG possessed the functions of inorganic phosphorus-dissolving,organic phosphorus-mineralizing,potassium dissolving and IAA-producing ability.Simultaneously possess three of these functions.[Conclusion]There are certain differences in the rhizosphere soil bacterial diversity and community composition of T.austromongolica in different conservation areas.Using culturable methods,11 core groups were identified,and two potential new species along with 27 strains exhibiting potential growth-promoting functions were obtained.This work enriches the resource base of high-quality functional bacterial strains with regional characteristics and independent intellectual property rights.