三种虫生真菌对番茄潜叶蛾幼虫的室内毒力测定及温室防治效果OA
Laboratory virulence assay and greenhouse control efficacy of three entomogenous fungi against the tomato leafminer,Tuta absoluta(Lepidoptera:Gelechiidae)larvae
[目的]本研究旨在明确3种虫生真菌对番茄潜叶蛾Tuta absoluta的杀虫效果,为番茄潜叶蛾的生物防治提供理论参考.[方法]采用点滴法,分别测定了不同浓度(1.0 × 106,1.0 × 107和1.0 × 108 cfu/mL)的 3 种虫生真菌(蜡蚧轮枝菌 Lecanicillium lecanii JMC-01、木贼镰刀菌 Fusarium equiseti JMF-01和雷斯青霉Penicillium raistrickii F-1紫外诱变菌株YB4)孢子悬浮液对番茄潜叶蛾1-2龄幼虫的室内毒力;根据室内毒力测定结果,用1.0 ×108 cfu/mL的3种虫生真菌孢子悬浮液在温室中采用随机区组法评价其对番茄潜叶蛾幼虫的防治效果.[结果]室内毒力结果表明,这3种虫生真菌对番茄潜叶蛾1-2龄幼虫均具有显著的毒力,在测试的最高浓度(1.0 ×108 cfu/mL)下,蜡蚧轮枝菌JMC-01、雷斯青霉F-1紫外诱变菌株YB4和木贼镰刀菌JMF-01孢子悬浮液引起的番茄潜叶蛾1-2龄幼虫的5 d累计校正死亡率分别为71.79％,61.54％和58.97％,对番茄潜叶蛾 1-2 龄幼虫的致死中时(median lethal time,LT50)值分别为 75.5166,89.1429 和96.4826 h.温室防治效果试验中,在1.0 ×108 cfu/mL浓度下,在第2次施药7 d后,蜡蚧轮枝菌JMC-01对番茄潜叶蛾幼虫的防治效果达到69.28％,雷斯青霉F-1诱变菌株YB4与木贼镰刀菌JMF-01对番茄潜叶蛾幼虫的防治效果分别达到了 52.69％和52.30％.[结论]3种虫生真菌对番茄潜叶蛾1-2龄幼虫均有一定的防治效果,其中蜡蚧轮枝菌JMC-01防治效果最高,具有作为番茄潜叶蛾生物防治剂的潜力.
[Aim]This study aims to clarify the insecticidal effects of three entomogenous fungi against Tuta absoluta,so as to provide a theoretical reference for its biological control.[Methods]Using the topical application method,the laboratory virulence of the spore suspensions of three entomogenous fungi(Lecanicillium lecanii JMC-01,Fusarium equiseti JMF-01,and the UV-mutated strain YB4 of Penicillium raistrickii F-1)at different concentrations(1.0 × 106,1.0 × 107 and 1.0 × 108 cfu/mL)against the 1st-2nd instar larvae of T.absoluta was tested.Based on the laboratory virulence test result,the spore suspensions of the three entomogenous fungi at the concentration of 1.0 × 108 cfu/mL were then used to evaluated their control efficacy against T.absoluta larvae in a greenhouse using a randomized block design.[Results]The laboratory virulence test results demonstrated that all the three entomogenous fungi exhibited significant virulence against the 1st-2nd instar larvae of T.absoluta.At the highest tested concentration(1.0 × 108 cfu/mL),the spore suspensions of L.lecanii JMC-01,the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 caused the 5-d cumulative corrected mortality rates of the 1st-2nd instar larvae of T.absoluta to reach 71.79％,61.54％and 58.97％,respectively.The median lethal time(LT50)values of the spore suspensions of L.lecanii JMC-01,the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 at the concentration of 1.0 × 108 cfu/mL to the 1st-2nd instar larvae of T.absoluta were 75.5166,89.1429 and 96.4826 h,respectively.In greenhouse control efficacy trials,at the concentration of 1.0 × 108 cfu/mL,L.lecanii JMC-01 demonstrated the control efficacy of 69.28％against T.absoluta larvae at 7 d after the 2nd application,whereas the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 achieved the control efficacy of 52.69％and 52.30％,respectively,against T.absoluta at 7 d after the 2nd application.[Conclusion]All the three entomogenous fungi have certain control efficacy against the 1st-2nd instar larvae of T.absoluta larvae,and L.lecanii JMC-01 shows the highest control efficacy,with the potential as a biocontrol agent.
田浩然;颜晓曦;李金鹏;白明;王新谱;洪波;贾彦霞
宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021||中国科学院动物研究所,动物多样性保护与有害动物防控全国重点实验室,北京 100101宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021
生物科学
番茄潜叶蛾虫生真菌毒力生物防治防效
Tuta absolutaentomogenous fungivirulencebiological controlcontrol efficacy
《昆虫学报》 2026 (2)
214-222,9
宁夏重点研发计划项目(2023BCF01045)
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