胃癌组织中miR-330-3p、IMP3表达水平与临床病理特征及预后的关系OA

Objective To investigate the correlation between the expression levels of miR-330-3p and insulin-like growth factor Ⅱ mRNA-binding protein-3(IMP3)in gastric cancer(GC)tissues with clinical pathological fea-tures and prognosis.Methods Seventy-five cases of gastric adenocarcinoma tissues and corresponding normal gastric mucosa tissues were selected and divided into GC group and paracancerous group.The expression levels of miR-330-3p and IMP3 in tissues were detected by quantitative real-time polymerase chain reaction(qRT-PCR),and the rela-tionship between their expression levels and the clinical pathological features of patients was analyzed.The Spearman rank correlation method was employed to evaluate the correlation between the expression levels of miR-330-3p and IMP3 in GC tissues.The miR-330-3p inhibitor and the miRNA-NC were transfected into the GC cell lines HGC27 and AGS using LipofectamineTM3000,respectively.The transfection efficiency of the plasmids and the expression level of IMP3 following miR-330-3p knockdown were assessed via qRT-PCR.Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of miR-330-3p and IMP3 and the prognosis of GC patients.Cox regres-sion model was used to analyze the influencing factors of poor prognosis in GC patients.Results Spearman correla-tion analysis showed that the expression level of miR-330-3p was negatively correlated with IMP3 in GC tissues(rs=-0.320,P<0.05).The expression level of miR-330-3p in GC tissues was significantly lower than that in adjacent tis-sues,and the difference was statistically significant(t=23.97,P<0.001);while the level of IMP3 was significantly higher than that in adjacent tissues,and the difference was statistically significant(t=16.91,P<0.001).The pro-portion of low expression of miR-330-3p and high expression of IMP3 in GC patients with T2-T4 stage,Ⅲ/Ⅳ stage,lymph node metastasis and distant metastasis was significantly higher than that in GC patients with T1 stage,Ⅰ/Ⅱstage,no lymph node metastasis and distant metastasis(P<0.05).Kaplan-Meier survival analysis showed that the incidence of good prognosis in GC patients with a high expression of miR-330-3p(75%)was higher than that of pa-tients with a low expression of miR-330-3p(5.6%),and the difference was statistically significant(χ2=29.759,P<0.001).The incidence of good prognosis in patients with a high IMP3 expression(13.9%)was lower than that in pa-tients with a low IMP3 expression(65.6%),and the difference was statistically significant(χ2=16.220,P<0.001).The incidence of good prognosis in GC patients with low miR-330-3p expression and high IMP3 expression(0%)was significantly lower than that in the other patients(60.5%),and the difference was statistically significant(χ2=26.905,P<0.001).Clinical stage Ⅲ/Ⅳ,low expression of miR-330-3p and high expression of IMP3 were risk factors for poor prognosis of GC patients(P<0.05).Conclusions The expression level of miR-330-3p exhibits a negative correlation with IMP3 in GC tissues.Furthermore,the downregulation of miR-330-3p and the upregulation of IMP3 are associated with the progression of malignant biological behavior and poor prognosis in gastric cancer pa-tients.These findings suggest that miR-330-3p and IMP3 may serve as potential therapeutic targets and biomarkers for predicting the prognosis of gastric cancer.