高药低酸型叶用银杏无性系综合评选OA

[Objective]This study aims to screen superior clones of Ginkgo biloba for leaf production with high medicinal components(total flavonoids and ginkgolides)and low ginkgolic acid content.[Method]The phenotypic traits(dry weight per leaf,leaf area,specific leaf dry weight)and medicinal component contents(total flavonoids,terpene lactones)of leaves from 46 ginkgo clones,as well as ginkgolic acid content,were systematically measured.Cluster analysis and principal component analysis based on membership functions were employed to comprehensively evaluate the 46 clones.[Result]There were significant differences in leaf phenotypic traits,medicinal component contents,and ginkgolic acid content among 46 ginkgo clones,indicating rich genetic diversity,with an average coefficient of variation of 13.8%.Based on the comprehensive evaluation using principal component analysis based on membership functions and combined with cluster analysis,six high-quality clones with high medicinal value and low ginkgolic acid content were selected,namely clones 25,34,1,5,45,and 3.These clones generally had higher specific leaf dry weight,higher contents of total flavonoids and terpene lactones,and moderate to low levels of ginkgolic acid.Among them,clone 34 had relatively larger leaf area,higher dry weight per leaf,and higher total medicinal component content,while its ginkgolic acid content was relatively low.[Conclusion]There are abundant variations in phenotypic traits,main medicinal components and ginkgolic acid content among different ginkgo clones.There is a certain synergistic change between medicinal components and ginkgolic acid,but the correlation between medicinal components is stronger.The results provides the possibility for the breeding of leaf-using ginkgo clones with high medicinal components and low ginkgolic acid.