胃癌中免疫相关基因SLC7A7的鉴定与初步验证OA

Objective To screen novel immune-related genes in gastric cancer through bioinfor-matics approaches,conduct preliminary experimental validation in gastric cancer cells,and elucidate the role of SLC7A7 in the immune microenvironment of gastric cancer via multi-omics integration.Methods Gastric cancer datasets were obtained from the GEO database,and differentially expressed genes were identified.Protein-protein interaction networks were analyzed using STRING software,while gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analy-ses were performed using Metascape.The correlation between gene expression and clinical characteris-tics was validated using TCGA data.The expression of SLC7A7 across various cancers was analyzed u-sing TIMER2.0.Survival analysis was conducted using Kaplan-Meier Plotter.The expression level of SLC7A7 in gastric cancer cell lines was validated by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Results SLC7A7 was highly expressed in gastric cancer and various other canc-ers,and was associated withpoor prognosis.SLC7A7 may exert its effects through the PI3K-Akt and AMPK signaling pathways.SLC7A7 was negatively correlated with memory B cells,naive B cells,monocytes and activated NK cells,while positively correlated with activated CD4+memory Tcells,γδ T cells,resting NK cells,unpolarized macrophages and M1/M2 macrophages.RT-qPCR results demonstra-ted high expression of SLC7A7 in five gastric cancer cell lines(HGC-27,NCI-N87,MKN-45,MKN-74 and AGS).Conclusion SLC7A7 is highly expressed in gastric cancer cell lines and is as-sociated with tumor immune infiltration and poor prognosis in gastric cancer patients.