血清微小RNA-26a、微小RNA-34a联合预测子宫肌瘤患者单孔腹腔镜术后复发的价值OA

Objective To analyze the changes in serum levels of microRNA-26a(miR-26a)and microRNA-34a(miR-34a)before and after single-port laparoscopic surgery for uterine fibroids(UF),as well as the value of their combined use in predicting postoperative recurrence.Methods A total of 200 UF patients who underwent single-port laparoscopic surgery were selected as study group,and 100 healthy women who underwent physical examinations during the same period were selected as control group.The serum levels of miR-26a and miR-34a were compared between the two groups.All patients in the study group were followed up for 1 year after surgery,and the postoperative recurrence status was recorded.The general information,as well as the preoperative and postoperative(at the 3rd month)serum miR-26a and miR-34a levels and their changes(denoted by △,representing the absolute difference value between preoperative and the 3rd postoperative month)were compared be-tween recurrent and non-recurrent patients,and the influencing factors for postoperative recurrence were screened.The value of △miR-26a and △miR-34a in predicting postoperative recurrence in UF patients was analyzed.Results The preoperative serum miR-26a and miR-34a levels in the study group were significantly lower than those in the control group(P＜0.05).In the study group,5 were lost to follow-up during the postoperative follow-up period,ultimately,195 patients completed the follow-up,of whom 51 patients experienced recurrence,with a recurrence rate of 26.15％(51/195).The number of UF in recurrent patients was significantly higher,the maximum diameter of UF was significantly larger,and the serum carbohydrate antigen-199(CA-199)and carcinoembryonic anti-gen(CEA)levels at 24 hours postoperatively were significantly higher in recurrent patients than those in non-recurrent patients(P＜0.05).At the 3rd month postoperatively,the serum miR-26a and miR-34a levels in both recurrent and non-recurrent patients in the study group were significantly higher than those preoperatively(P＜0.05).The preoperative and postoperative(at the 3 rd month)serum miR-26a and miR-34a levels,as well as △miR-26a and △miR-34a were significant-ly lower in recurrent patients than those in non-recurrent patients(P＜0.05).The number of UF,serum CA-199 and CEA levels at 24 hours postoperatively,△miR-26a and △miR-34a were all in-fluencing factors for postoperative recurrence in UF patients(P＜0.05).The area under the curve(AUC)for predicting postoperative recurrence using the combination of △miR-26a and △miR-34a was 0.847,which was greater than the AUCs for predicting recurrence using △miR-26a,△miR-34a,the number of UFs as well as serum CA-199 and CEA levels at 24 hours postoperatively alone(P＜0.05).Conclusion The serum miR-26a and miR-34a levels are significantly decreased in UF pa-tients,and their changes before and after surgery are closely related to postoperative recurrence.The combined predictive efficacy of △miR-26a and △miR-34a for postoperative recurrence is signifi-cantly superior to that of traditional tumor markers.