尿液蛋白质组学和代谢组学在慢性低氧模型小鼠中的动态变化OA

Objective To detect changes in the metabolome and proteome of urine in a mouse model of chronic hy-poxia and to explore the correlation between the urinary proteome and metabolome.Methods Six C57BL/6J male mice were placed in an oxygen chamber with 14％oxygen concentration for 5 weeks of hypoxic exposure.Mouse urine was collected weekly with a control group established simultaneously.Non-targeted proteomics and metabolo-mics techniques were employed for dynamic analysis of urine samples.Results Compared to the control group,the urine of chronic hypoxia mice contained 458 differentially expressed proteins and 718 differential metabolites.These proteins and differential metabolites were functionally enriched primarily in glycolysis pathway,lysosomal pathway,and cholesterol synthesis pathway.Joint analysis of proteomics and metabolomics revealed that proteins with high correlation to differential metabolites were mainly enriched in PI3K-AKT,complement and coagulation cascades,and cell cycle pathways.Conclusions Urinary proteomics and metabolomics may reflect the changes induced by chronic hypoxic stimulation.