轮作和连作对植烟土壤微生物多样性与群落结构的影响OA

This study aims to investigate the effect of various agricultural practices on the microbial community composition in tobacco soil.Soil samples were collected from plots under rotational and continuous tobacco cultivation,and microbial communities were analyzed by high-throughput sequencing.The α-diversity indexes showed that the microbial diversity in the soil of rotational cultivation was higher than that in the soil of continuous cultivation.Principal coordinate analysis clear distinguished the bacterial community structures between the two cultivation systems.At the phylum level,Proteobacteria and Ascomycota were dominant in the soil samples of rotational and continuous cultivation.At the genus level,Anaeromyxobacter and Galactomyces were more abundant.Notably,Boothiomyces was identified as the potential biomarker for continuous cultivation,whereas Penicillium was identified as that for rotational cultivation.Furthermore,available potassium and alkali-hydrolyzable nitrogen were the main influencing factors for the bacterial and fungal communities,respectively,in the soil of the two cultivation systems.These results indicated that implementing rotational cultivation could enhance the microbial diversity in the soil.