基因型对辣椒花药培养效果的影响OA

This study utilized 48 distinct genotypes of Capsicum annuum L.cultivars for anther embryoid induction and regenerated plantlet culture test,aiming to screen the genotypes with superior androgenic competence and unveil the morphogenetic process of embryoids.The results revealed that the coefficients of variation of anther swelling rate,browning rate,embryoid induction rate,and plantlet regeneration rate reached 70.63%,28.60%,101.27%,and 87.86%,respectively,among the 48 genotypes.The anther swelling rate had a significantly positive correlation with browning rate and extremely significantly positive correlations with embryoid induction rate and plantlet regeneration rate.The browning rate showed extremely significant positive correlations with embryoid induction rate and plantlet regeneration rate.Furthermore,the embryoid induction rate and plantlet regeneration rate exhibited an extremely significantly positive correlation.The screening process identified genotype 22Y19 as an outstanding androgenic performer,achieving an embryoid induction rate of 20.14%and a plantlet regeneration rate of 42.25%.Developmental tracking revealed that embryogenic progression of 22Y19 through four distinct morphological stages:globular,heart-shaped,torpedo,and cotyledonary embryos,culminating in complete plant formation.This genotype could not only be used as a bridge parent to enhance the efficiency of anther culture for genotypes with low induction rates but also as a key experimental material for deciphering the molecular mechanism of embryoid development.