基于SSR和ISSR分子标记的华南地区香稻种质资源遗传多样性分析OA

This study systematically analyzed the genetic diversity of 20 aromatic rice germplasm accessions from South China based on SSR and ISSR markers.The results showed that the amplification with eight pairs of SSR primers produced 63 bands,among which 41 bands showed polymorphism,with an average polymorphism rate of 63.07%.The genetic similarity coefficients ranged from 0.382 9 to 0.969 6,with a mean value of 0.676 3,indicating moderate polymorphism.Five ISSR primers produced 38 bands,among which 15 bands exhibited polymorphism,with an average polymorphism rate of 41.80%.The genetic similarity coefficients ranged from 0.714 2 to 0.985 9,with a mean value of 0.850 1,indicating low polymorphism.SSR markers detected higher polymorphism than ISSR markers,being more suitable for the genetic diversity analysis of aromatic rice.Cluster analysis classified the accessions into three major groups.Group I contained 17 accessions,while Groups II and III consisted of 1 and 2 accessions,respectively.SSR and ISSR markers were consistent in the classification of most accessions but had differences in the classification of some accessions(such as'Xiangxiangsimiao'and'Meixiangzhan'),which indicated that the genetic basis of cultivated rice was narrow and there were genetic differences among accessions.