木薯普通花叶病毒CsCP与MeGRXC3互作分析OA

Cassava common mosaic virus(CsCMV)is the primary agent causing cassava mosaic dis-ease.Its capsid protein(CP)plays a crucial role in virus-plant interactions during infection.Glutaredoxins(GRXs)are thiol oxidoreductases with low molecular weight,among which CC-type GRXs are key regu-lators of resistance to plant disease.Given that CP is a key pathogenicity factor of CsCMV involved in mod-ulating host immune responses,this article investigated whether CsCP interacts with MeGRXC3 to syner-gistically regulate redox homeostasis and thereby modulate viral infection.The yeast two-hybrid(Y2H),bi-molecular fluorescence complementation(BiFC),and subcellular localization assays were used to examine the interaction between CsCP and MeGRXC3.The results showed that there was an interaction between cassava MeGRXC3 and CsCP in yeast cells.Green fluorescence appeared at 488 nm after co-expressing Me-GRXC3 and CsCP in Nicotiana benthamiana epidermal cells,confirming the interaction between CsCP and MeGRXC3 in plant cells.CsCP and MeGRXC3 exhibited co-localization in Nicotiana benthamiana epi-dermal cells.It is indicated that there is an interaction between CsCP and MeGRXC3.It will lay the ground-work for further elucidating the molecular mechanisms by which CP facilitates the infection of CsCMV.